Sb bicolor PI329311 (IS 11069) Assembly and Gene Annotation

About PI 329311

PI 329311 is a durra-type sorghum from Ethiopia that is one of the parental lines in the CP-NAM population (Boatwright et al. 2021). It was selected for this population because it was representative of East African genetic diversity. It is also known by the ICRISAT accession number IS 11069. This line was a top performer in terms of cellulosic biomass production in a series of trials performed at Clemson University.

Germplasm synonyms: * GRIN: PI 329311 * ICRISAT: IS 11069

Population panels: * CP-NAM - 12 accessions (Boatwright et al, 2021; Boatwright et al, 2022; Kumar et al, 2022)

Assembly

The preliminary assembly was constructed using Canu and 30x coverage Pacbio long read data and then aligned to the reference (BTx623) using MUMmer (Voelker et al, 2022). Contigs were sorted using coordinate data from MUMmer and code developed by the Cooper Lab at UNCC (https://github.com/cponce-uncc/bioinformatics_tools). Contigs under 50k base pairs were discarded, as were contigs that were manually determined to not clearly map to a position after sorting. Final assembly statistics are as follows:

Final Contig Count: 2542

Final Base Pair Count: 595430490

Percent Coverage of Reference: 81.566%

Annotation

Gene annotations were built by the Ware Lab in CSHL using a pan-gene index from reference gene models and propagated to representative assemblies using the Liftoff tool and further updating their structure using PASA tool (Voelker et al, 2022)

References

Boatwright JL, Brenton ZW, Boyles RE, Sapkota S, Myers MT, Jordan KE, Dale SM, Shakoor N, Cooper EA, Morris GP, Kresovich S. 2021. Genetic characterization of a Sorghum bicolor multiparent mapping population emphasizing carbon-partitioning dynamics. G3 (Bethesda). 11(4):jkab060. PMID: 33681979. doi: 10.1093/g3journal/jkab060.

Boatwright JL, Sapkota S, Jin H, Schnable JC, Brenton Z, Boyles R, Kresovich S. 2022. Sorghum Association Panel whole-genome sequencing establishes cornerstone resource for dissecting genomic diversity. Plant J. PMID: 35653240. doi: 10.1111/tpj.15853.

Kumar N, Brenton ZW, Myers MT, Boyles RE, Sapkota S, Boatwright JL, Cox WA, Jordan KE, Kresovich S. 2022. Registration of the sorghum carbon‐partitioning nested association mapping (CP‐NAM) population. Journal of Plant Registrations. 1-8. doi: 10.1002/plr2.20229.

Voelker WG, Krishnan K, Chougule K, Alexander LC, Lu Z, Olson A, Ware W, Songsomboon K, Ponce C, Brenton ZW, Boatwright JL, Cooper EA. 2022. Ten new high-quality genome assemblies for diverse bioenergy sorghum genotypes. bioRxiv. doi: 10.1101/2022.09.26.509520.

Links

GRIN: PI 329311

Image

https://npgsweb.ars-grin.gov/gringlobal/ImgDisplay?id=1244841

Image source: The GRIN database